Document Details

Document Type : Article In Journal 
Document Title :
EFFECT OF NICOTINE AND ESTROGEN ON INTERLEUKIN-6 RELEASE FROM OSTEOBLASTS
EFFECT OF NICOTINE AND ESTROGEN ON INTERLEUKIN-6 RELEASE FROM OSTEOBLASTS
 
Document Language : English 
Abstract : Smoking may interfere with the effects of estrogen leading to increases in bone resorptive cy-tokines such as interleukin-6 (IL-6). Our present study investigates the effect of nicotine and 17ß estradiol on IL-6 production by mice calvaria osteoblasts. Osteoblastic enriched cells were isolated by sequential collagenase digestion of neonatal mouse calvaria. A specific immunoassay for IL-6 was used to measure the cytokine levels in the media after 24 hours of incubation of the cells with these agents. ANOYA and Fisher's protected multiple comparison were utilized for statistical anal-yses. It was found that nicotine concentrations of 100 μg/ml significantly increased (P=O.OOO1) IL-6 release by osteoblasts compared to negative controls. On the other hand, 17ß estradiol at concentrations of 10-7M inhibited significantly (P=O.OOOl) lL-6 release compared to negative controls. In conclusion, these results suggest that nicotine and estradiol have opposing effects on IL-6 production by osteoblastic cells. These effects may explain, in part, the observation of diminished benefit of hormone replacement therapy on bone loss in postmenopausal women who smoke 
ISSN : 1 
Journal Name : Egyption Dental Journal 
Volume : 47 
Issue Number : 2 
Publishing Year : 2001 AH
2001 AD 
Article Type : Article 
Added Date : Saturday, June 21, 2008 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ندى الغرابEl –Ghorab, Nada InvestigatorDoctoratenalgarab@kau.edu.sa

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