Document Details Document Type : Article In Journal  Document Title : "Synapse elimination in botulinum toxin-paralysed rat muscles" "Synapse elimination in botulinum toxin-paralysed rat muscles"   Document Language : English  Abstract : In order to determine the fate of the superfluous neuromuscular junctions (NMJs) formed during the course of botulinum toxin (BoTx)-induced paralysis, we have quantified the change in the total length of the nerve muscle contact area(s) following BoTx injection into rat calf muscles. The results indicate that: (1) at least some of the superfluous NMJs are eliminated following muscle recovery; (2) synapse elimination is a slow process, as 4 months after recovery it was not yet complete; (3) muscles with different content of type I and II fibers follow a different time course during synapse formation and elimination. We further investigated the possibility that the neural cell adhesion molecule (NCAM) would be the element whose loss from the NMJ might play a role in synapse elimination. Using immunofluorescence and immunoelectron microscopy we show that NCAM is exclusively localized between nerve terminals and Schwann cells and not between nerve terminals and muscle. This localization was maintained throughout paralysis and following recovery, suggesting that NCAM does not play a role in synapse elimination.  ISSN : 16135345  Journal Name : Journal of Neurology  Volume : 241  Issue Number : 6  Publishing Year : 1994 AH 1994 AD  Article Type : Article  Added Date : Sunday, March 28, 2010  Researchers Researcher Name (Arabic) Researcher Name (English) Researcher Type Dr Grade Email شريف حسن Hassan, Sherif Researcher Doctorate   Files File Name Type Description  26217.1 1   Back To Researches Page